Surrogate approach to determine heavy metal loads in a moss species – Barbula lambaranensis

AbstractBiomonitoring using a moss species Barbula lambaranensis is an economical method for continuous assessment of atmospheric metal pollution. However, frequent measurement of common heavy metals such as Zn, Cd, Cr, Pb, Cu and Ni in moss can be costly for monitoring large areas. Thus, the aim of the study was to use the surrogate approach to reduce the number of heavy metals required for monitoring. The study found that the Zn load in moss was higher; Pb, Cu and Ni loads were moderate; while Cd and Cr were relatively lower across the study sites. Further, the following surrogates were identified based on PCA: Cu for Cr; Pb for Cd, Cu and Ni; and Cu and Pb for Zn. Quantitative relationships between surrogate loads and the loads of other heavy metals were developed by performing Multiple Linear Regression on a data set constructed using a four level full factorial design. The equations had a relative prediction error and standard error of cross validation below 25% and 1.5%, respectively, indicating that the equations are accurate. However, the cross validated coefficient of determination is relatively low suggesting that the precision of prediction using the equations is low, possibly due to the influence of factors such as climatic conditions on bioaccumulation of heavy metals by moss. Nevertheless, the developed equations can be useful for preliminary investigations

A Floristic Assessment of Woody Plant Diversity in Secondary Forest, Ile-Ife Nigeria: A Proactive Step to Monitoring the Diversity Loss and Degradation

Loss of biodiversity has been recognized as one of the main threats to the forest ecosystem. This study examined the diversity and conservation status of woody species in the secondary forest of Obafemi Awolowo University, Ile-Ife, Nigeria using six permanent plots. All woody species were completely enumerated, and identified; their girths at breast height were measured at 1.3 m above buttress. Biodiversity status was assessed using Shannon-Wiener diversity, evenness of distribution, Sorrenson index of similarity and species importance value index. There were 77 tree species distributed into 62 genera and 30 families. The most abundant species were Celtis zenkeri and Lecaniodiscus cupanioides, while the most abundant families were Moraceae, Euphorbiaceae, and   Fabaceae. The tree species density ranged from 508 – 1132 plants per hectare. The Shannon-Weiner diversity index (1.83 to 3.50), evenness (0.483 – 0.741) and the basal area (6.06 – 25.73 m2 ha-1) were high in the forest. However, Celtis mildbraedii is critically endangered, while Garcina kola, Mallotus oppositifolus and Ricinodendron heudelotii are vulnerable. This study provides baseline diversity data for the conservation of woody species in the forest and concludes that this secondary forest is a potential biodiversity hub if properly managed. It will also play a key role in fashioning out realistic, appropriate, and effective conservation strategies that will enhance the restoration and management of the floristic composition of the secondary forest

Health system barriers influencing timely breast cancer diagnosis and treatment among women in low and middle-income Asian countries: evidence from a mixed-methods systematic review.

Globally, breast cancer is the most common cancer type and the leading cause of cancer mortality among women in developing countries. A high prevalence of late breast cancer diagnosis and treatment has been reported predominantly in Low- and Middle-Income Countries (LMICs), including those in Asia. Thus, this study utilized a mixed-methods systematic review to synthesize the health system barriers influencing timely breast cancer diagnosis and treatment among women in Asian countries. We systematically searched five electronic databases for studies published in English from 2012 to 2022 on health system barriers that influence timely breast cancer diagnosis and treatment among women in Asian countries. The review was conducted per the methodology for systematic reviews and reported following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, while health system barriers were extracted and classified based on the World Health Organization (WHO)'s Health Systems Framework. The mixed-methods appraisal tool was used to assess the methodological quality of the included studies. Twenty-six studies were included in this review. Fifteen studies were quantitative, nine studies were qualitative, and two studies used a mixed-methods approach. These studies were conducted across ten countries in Asia. This review identified health systems barriers that influence timely breast cancer diagnosis and treatment. The factors were categorized under the following: (1) delivery of health services (2) health workforce (3) financing for health (4) health information system and (5) essential medicines and technology. Delivery of health care (low quality of health care) was the most occurring barrier followed by the health workforce (unavailability of physicians), whilst health information systems were identified as the least barrier. This study concluded that health system factors such as geographical accessibility to treatment, misdiagnosis, and long waiting times at health facilities were major barriers to early breast cancer diagnosis and treatment among Asian women in LMICs. Eliminating these barriers will require deliberate health system strengthening, such as improving training for the health workforce and establishing more healthcare facilities. [Abstract copyright: © 2022. The Author(s).

Enthesis tissue engineering: biological requirements meet at the interface

Tendon-to-bone interface (enthesis) exhibits a complex multiscale architectural and compositional organization maintained by a heterogeneous cellular environment. Orthopedic surgeons have been facing several challenges when treating tendon pullout or tear from the bony insertion due to unsatisfactory surgical outcomes and high retear rates. The limited understanding of enthesis hinders the development of new treatment options toward enhancing regeneration. Mimicking the natural tissue structure and composition is still a major challenge to be overcome. In this review, we critically assess current tendon-to-bone interface tissue engineering strategies through the use of biological, biochemical, or biophysical cues, which must be ultimately combined into sophisticated gradient systems. Cellular strategies are described, focusing on cell sources and cocultures to emulate a physiological heterotypic niche, as well as hypoxic environments, alongside with growth factor delivery and the use of platelet-rich hemoderivatives. Biomaterial design considerations are revisited, highlighting recent progresses in tendon-to-bone scaffolds. Mechanical loading is addressed to uncover prospective engineering advances. Finally, research challenges and translational aspects are considered. In summary, we highlight the importance of deeply investigating enthesis biology toward establishing foundational expertise and integrate cues from the native niche into novel biomaterial engineering, aiming at moving today's research advances into tomorrow's regenerative therapies.Authors thank the support from the European Union Framework Programme for Research and Innovation HORIZON2020 [TEAMING Grant agreement No 739572 - The Discoveries CTR]; FCT–Fundação para a Ciência e a Tecnologia for the PhD grant of IC [PD/BD/128088/2016]; the Project NORTE-01-0145-FEDER-000021:“Accelerating tissue engineering and personalized medicine discoveries by the integration of key enabling nanotechnologies, marine-derived biomaterials and stem cells”, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and the ERC Consolidator grant of ME [ERC-2017-CoG-772817]

Enhancing moringa nutritive value for impoverished populations and healthy wellbeing through proper nutrient enrichment

Taking into account the many cases of malnutrition related deaths in especially sub-Saharan Africa, the current research effort was conceived with the objective of appraising the nutritive potential of Moringa oleifera L. plant through the evaluation of proper agronomic practice that will enhance its nutritive potential. Measured quantity of dried roots, stem, and leaves portions of 5 months old Moringa oleifera L. seedlings, grown under various applied phosphorus (P) and potassium (K) rates were analysed for essential and non-essential amino acids content, total antioxidants, secondary metabolites (phenolics and flavonoids), and mineral nutrient elements. The high-performance liquid chromatography (HPLC) analysis revealed the presence of 9 essential and 7 non-essential amino acids, with a range of 4.24 to 10.04 gkg-1 recorded for leucine, threonine, histidine, glutamic acid, aspartic acid, and glycine. The 2,2ꞌ- diphenyl-1-picrylhydrazyl (DPPH) and the ferric reducing antioxidant power (FRAP) assay revealed strong antioxidants presence (up to 68% DPPH scavenging activity and a high level FRAP potential of 2500 mg ascorbic acid equivalent (AAE) / 100g dry weight (DW). Folin-Ciocalteu’s and aluminium chloride calorimetric assay revealed high levels of secondary metabolites (ranging from 1000 to 1350 mg gallic acid equivalent (GAE)/100g DW total phenolics and up to 2400 mg quercetin equivalent (QE)/1mg DW total flavonoids), and the nitric acid wet digestion procedure revealed a lot of mineral nutrient elements (with significant values of up to 19.13 g kg-1 maximum content acquired from calcium (Ca) and up to 9.90 g kg-1 recorded for iron (Fe). These nutritive components are noted to be highest in the leaves portion, followed by the roots, and then the stem parts. Based on its recognised rich base nutritive content, Moringa oleifera L. is a tree plant of nutraceuticals importance for impoverished communities

Terminology for Achilles tendon related disorders

The terminology of Achilles tendon pathology has become inconsistent and confusing throughout the years. For proper research, assessment and treatment, a uniform and clear terminology is necessary. A new terminology is proposed; the definitions hereof encompass the anatomic location, symptoms, clinical findings and histopathology. It comprises the following definitions: Mid-portion Achilles tendinopathy: a clinical syndrome characterized by a combination of pain, swelling and impaired performance. It includes, but is not limited to, the histopathological diagnosis of tendinosis. Achilles paratendinopathy: an acute or chronic inflammation and/or degeneration of the thin membrane around the Achilles tendon. There are clear distinctions between acute paratendinopathy and chronic paratendinopathy, both in symptoms as in histopathology. Insertional Achilles tendinopathy: located at the insertion of the Achilles tendon onto the calcaneus, bone spurs and calcifications in the tendon proper at the insertion site may exist. Retrocalcaneal bursitis: an inflammation of the bursa in the recess between the anterior inferior side of the Achilles tendon and the posterosuperior aspect of the calcaneus (retrocalcaneal recess). Superficial calcaneal bursitis: inflammation of the bursa located between a calcaneal prominence or the Achilles tendon and the skin. Finally, it is suggested that previous terms as Haglund’s disease; Haglund’s syndrome; Haglund’s deformity; pump bump (calcaneus altus; high prow heels; knobbly heels; cucumber heel), are no longer used

Measuring the extent the environmental pollution of the waters of the Diwani River some trace elements resulting from Diwani textile factory using some spectroscopic methods

The concentrations of some trace elements cobalt, nickel, lead, mercury cadmium, copper and Iron as well as pH value and the conductivity were determined in the samples of water which input and output (waste) from AL-Diwani textile factory and the effect of it on the AL-Diwani river water which is nearest from the factory through one month. The concentrations of above elements were determined in the samples by two methods. The flame Atomic absorption spectrophotometry and the spectrometry method which is satiable for each ion and making Analytical comparative between them from some analytical values like correlation coefficient(r), relative standard error Erel%, recovery value Re% and relative standard deviation RSD% for each ion. It is found that the first method is more accuracy for measuring the all elements concentrations except measuring of Iron. From results we show that the Lead element is more concentration among other elements which was the middle concentration was 3.3 ppm. The cobalt element is less concentration from other elements which was the middle of it concentration was 0.043 ppm in the waste water samples and other elements have concentration between them in the different samples. We show the increasing on the acidity and Conductivity for waste samples as comparative with the other samples which were pH between 6.5-7.2 and the Conductivity from the instrument directly in between 1210-1913 mS∙cm-1 and the measuring was making at the room temperature